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Literature summary extracted from

  • Siripurkpong, P.; Yuvaniyama, J.; Wilairat, P.; Goldberg, D.E.
    Active Site Contribution to Specificity of the Aspartic Proteases Plasmepsins I and II (2002), J. Biol. Chem., 277, 41009-41013.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.39 engineering a plasmepsin II with the active site surface of plasmepsin I and expression in Escherichia coli Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
3.4.23.39 additional information engineering a plasmepsin II with the active site surface of plasmepsin I largely maintains the plasmepsin II substrate and inhibitor specificity Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.38 pepstatin
-
 Plasmodium falciparum
3.4.23.38 SC-50083 IC50: 0.000132 mM Plasmodium falciparum
3.4.23.39 pepstatin
-
 Plasmodium falciparum
3.4.23.39 SC-50083 IC50: 0.0003467 mM Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.38 0.000917
-
 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid pH 5.0, 37°C Plasmodium falciparum
3.4.23.39 0.000489
-
 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid pH 5.0, 37°C Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.38 Hemoglobin + H2O Plasmodium falciparum enzyme is involved in initial steps of Plasmodium hemoglobin degradation ?
-
 ?
3.4.23.39 Hemoglobin + H2O Plasmodium falciparum enzyme is involved in initial steps of Plasmodium hemoglobin degradation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.38 Plasmodium falciparum
-
-
-
3.4.23.39 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.38
-
 Plasmodium falciparum
3.4.23.39
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.38 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe + Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O
-
 Plasmodium falciparum ?
-
 ?
3.4.23.38 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O
-
 Plasmodium falciparum ?
-
 ?
3.4.23.38 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O
-
 Plasmodium falciparum 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe + Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid
-
 ?
3.4.23.38 Hemoglobin + H2O enzyme is involved in initial steps of Plasmodium hemoglobin degradation Plasmodium falciparum ?
-
 ?
3.4.23.39 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O
-
 Plasmodium falciparum ?
-
 ?
3.4.23.39 Hemoglobin + H2O enzyme is involved in initial steps of Plasmodium hemoglobin degradation Plasmodium falciparum ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.38 0.473
-
 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid pH 5.0, 37°C Plasmodium falciparum
3.4.23.39 2.31
-
 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid pH 5.0, 37°C Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.23.38 0.000000565
-
 pepstatin pH 5.0, 37°C Plasmodium falciparum
3.4.23.39 0.000000037
-
 pepstatin pH 5.0, 37°C Plasmodium falciparum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.23.38 0.000132
-
 IC50: 0.000132 mM Plasmodium falciparum SC-50083
3.4.23.39 0.0003467
-
 IC50: 0.0003467 mM Plasmodium falciparum SC-50083